Radiology

Advancing Tumor Budding Detection With Fourier Ptychography Microscopy

Ethan Littlefield

05 Jul 2025 — 1 min read

PMCID: PMC12175198

PMID: 40530892

DOI: 10.1002/cam4.70989

Journal: Cancer medicine

Publication Date: 2025-6-18

Authors: Su Y, Sun R, Wang Y, xi Y, Zhang S

Key Points

FPM enables digital refocusing and marker removal without physical slide manipulation
100% diagnostic accuracy achieved using a simplified microscopy approach
Potential to reduce pathologist workload and minimize technical diagnostic barriers

Summary

This study introduces Fourier ptychographic microscopy (FPM) as an innovative diagnostic imaging technique for pathological assessment of tumor budding in colorectal cancer. By eliminating the need for mechanical focusing and objective switching, FPM offers a streamlined approach to pathological slide examination that maintains diagnostic accuracy while reducing technical complexity.

The research demonstrated FPM's capability to provide high-resolution, digitally refocused images with 100% diagnostic accuracy when compared to conventional microscopy. Critically, the technique allows pathologists to overcome common challenges such as slide thickness variations, surface contamination, and manual marking interference, all while maintaining precise tumor budding identification across 600 pathological sections.

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