Radiology

Advancing Tumor Budding Detection With Fourier Ptychography Microscopy

Ethan Littlefield

25 Jun 2025 — 1 min read

PMCID: PMC12175198

PMID: 40530892

DOI: 10.1002/cam4.70989

Journal: Cancer medicine

Publication Date: 2025-6-18

Authors: Su Y, Sun R, Wang Y, xi Y, Zhang S

Key Points

FPM enables digital refocusing and marker removal without physical slide manipulation
100% diagnostic accuracy achieved using a simplified microscopy approach
Potential to reduce pathologist workload and minimize technical diagnostic barriers

Summary

This study introduces Fourier ptychographic microscopy (FPM) as an innovative diagnostic imaging technique for pathological assessment of tumor budding in colorectal cancer. By eliminating the need for mechanical focusing and objective switching, FPM offers a streamlined approach to pathological slide examination that maintains diagnostic accuracy while reducing technical complexity.

The research demonstrated FPM's capability to provide high-resolution, digitally refocused images with 100% diagnostic accuracy when compared to conventional microscopy. Critically, the technique allows pathologists to overcome common challenges such as slide thickness variations, surface contamination, and manual marking interference, all while maintaining precise tumor budding identification across 600 pathological sections.

Status and associated factors of psychological resilience of Chinese medical aid team members under public health emergencies in a cross-sectional study

PMCID: PMC12173397 PMID: 40526630 DOI: 10.1371/journal.pone.0324952 Journal: PloS one Publication Date: 2025-6-17 Authors: Wei X, Liang Y, Huang J Key Points * Psychological resilience is significantly influenced by professional experience, family support, and cultural adaptation * 53.9% of CMATMs reported experiencing loneliness, which negatively impacted psychological resilience

Structured light imaging mesoscopy: detection of embedded morphological changes in superficial tissues

PMCID: PMC12175002 PMID: 40534904 DOI: 10.1117/1.JBO.30.6.065001 Journal: Journal of biomedical optics Publication Date: 2025-6-18 Authors: Parsanasab M, Mehendale AM, Karrobi K, Roblyer D, Venugopalan V Key Points * SLIM provides a novel, non-invasive method for detecting subsurface tissue microstructural changes * Optimal imaging parameters vary significantly

Experimental evolution of a pathogen confronted with innate immune memory increases variation in virulence

PMCID: PMC12176410 PMID: 40532126 DOI: 10.1371/journal.ppat.1012839 Journal: PLoS pathogens Publication Date: 2025-6-18 Authors: Korša A, Baur M, Schulz NK, Anaya-Rojas JM, Mellmann A, et al. Key Points * Immune priming creates selective pressure that increases pathogen virulence variability without compromising host defense mechanisms * 96.6% probability of

Long‐Term or Recurrent Antibiotic Use in Early Life and the Risk of Type 2 Diabetes: A Population‐Based Prospective Cohort and a Case–Control Study

PMCID: PMC12176498 PMID: 40533417 DOI: 10.1111/1753-0407.70113 Journal: Journal of diabetes Publication Date: 2025-6-18 Authors: Li Z, He Q, He X, Xing X, Fu S, et al. Key Points * Long-term or recurrent antibiotic use during childhood may significantly increase type 2 diabetes risk * 26% higher hazard ratio for